Role of prostaglandins and leukotrienes in volume regulation by Ehrlich ascites tumor cells
- 1 October 1987
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 98 (3), 247-256
- https://doi.org/10.1007/bf01871187
Abstract
Summary PGE2 and LTC4 syntheses in Ehrlich ascites cells were measured by radioimmunoassay. Hypotonic swelling results in stimulation of the leukotriene synthesis and a concomitant reduction in the prostaglandin synthesis. If the cells have access to sufficient arachidonic acid there is a parallel increase in the synthesis of both leukotrienes and prostaglandins following hypotonic exposure. PGE2 significantly inhibits regulatory volume decrease (RVD) following hypotonic swelling in Na-containing medium but not in Na-free media, supporting the hypothesis that the effect of PGE2 is on the Na permeability. PGE2 also had no effect on RVD in Na-free media in the presence of the cation ionophore gramicidin. Since the Cl permeability becomes rate limiting for RVD in the presence of gramicidin, whereas the K permeability is rate limiting in its absence, it is concluded that PGE2 neither affects Cl nor K permeability. Addition of LTD4 accelerates RVD and since the K permeability is rate limiting for RVD this shows that LTD4 stimulates the K permeability. Inhibition of the leukotriene synthesis by nordihydroguaiaretic acid inhibits RVD even when a high K conductance has been ensured by the presence of gramicidin. It is, therefore, proposed that an increase in leukotriene synthesis after hypotonic swelling is involved also in the activation of the Cl transport pathway.Keywords
This publication has 31 references indexed in Scilit:
- Effect of arachidonic acid, fatty acids, prostaglandins, and leukotrienes on volume regulation in Ehrlich ascites tumor cellsThe Journal of Membrane Biology, 1987
- Mechanism for leukotriene C4 stimulation of chloride transport in corneaThe Journal of Membrane Biology, 1986
- Separate, Ca2+-activated K+ and Cl− transport pathways in Ehrlich ascites tumor cellsThe Journal of Membrane Biology, 1986
- ARACHIDONIC ACID METABOLISMAnnual Review of Biochemistry, 1986
- Volume-induced increase of K+ and Cl− permeabilities in Ehrlich ascites tumor cells. Role of internal Ca2+The Journal of Membrane Biology, 1984
- Leukotrienes: Mediators of Immediate Hypersensitivity Reactions and InflammationScience, 1983
- The relationship between urinary prostaglandin excretion rates and urine flow in conscious rats. Evaluation of the radioimmunoassay by gas chromatography‐mass spectrometryActa Physiologica Scandinavica, 1983
- Volume-induced increase of anion permeability in human lymphocytes.The Journal of general physiology, 1982
- A study of the effect of stimulated endogenous prostaglandin synthesis on urine flow, osmolar excretion rate, and renin release in hydropenic and saline loaded, anesthetized ratsActa Physiologica Scandinavica, 1981
- Calmodulin stimulates human platelet phospholipase A2Biochemical and Biophysical Research Communications, 1979