Glucuronidation of bile acids in human liver, intestine and kidney
- 23 September 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 189 (2), 183-187
- https://doi.org/10.1016/0014-5793(85)81020-6
Abstract
The activities of UDP-glucuronyl transferase(s) in homogenates and microsomal preparations of human liver, kidney and intestine were tested with hyodeoxycholic acid (HDC). The various kinetic parameters of the UDC-glucuronidation were determined from time course experiments. In both liver and kidney preparations, HDC underwent a very active metabolic transformation: liver Km = 78 μM, Vmax = 3.3 nmol·min −1mg −1 protein; kidney Km = 186 μM, Vmax = 9.9 nmol·min −1.mg −1 protein. To our knowledge this is the first observation of both an extensive and comparable bile acid glucuronidation occurring in renal and hepatic tissuesKeywords
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