This is a further and somewhat more detailed report on the character of cultures of tubercle bacilli grown in submerged form in simple media containing the wetting agent "Tween 80". In such cultures the organisms grow much as ordinary bacteria in a fluid medium, eventually settling out in the bottom of the culture flask as a somewhat mucoid sediment. Undoubtedly this is the most interesting phenomenon in the bacteriology of tuberculosis to have appeared in many yrs. In the present paper the authors show that organisms so grown retain the same biologic and im-munologic properties shown when grown on the usual media used in the past. The chemical-nutritional factors involved in the growth of the bacilli are considered most briefly, a field of investigation which may be greatly amplified in the next few yrs.