Ref cell hydrolases: Glycosidase activities in human erythrocyte plasma membranes
- 1 December 1971
- journal article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 4 (1), 113-123
- https://doi.org/10.1007/bf02431965
Abstract
Human erythrocyte plasma membranes were found to contain the following glycosidases: α- and β-glucosidase, α- and β-galactosidase, α- and β-fucosidase, β-N-acetylglucosaminidase, β-N-acetylgalactosaminidase, β-xylosidase and α-mannosidase. All the enzymes except β-fucosidase had activity interpreted to be on the external surface of the plasma membrane. The enzymes had optimum pH values of 5.2 to 5.0 and temperatures of 37 to 40°C. The enzymes were not greatly activated by divalent cations but Hg++ and Pb++ were inhibitory. The enzyme extract of the human erythrocyte plasma membranes liberated carbohydrate from intact red cells, which lead to the speculation that the glycosidases might function to modify the erythrocyte plasma membrane.Keywords
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