Sequence and heterogeneity in the 5S RNA gene cluster of Drosophila melanogaster
Open Access
- 11 February 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (3), 441-451
- https://doi.org/10.1093/nar/8.3.441
Abstract
The sequence of the entire 5S RNA gene of Drosophila melanogaster was determined by sequencing collectively 23 copies contained in a cloned fragment of Drosophila DNA and by sequencing individually four subcloned gene copies. A repetitive heptamer (GCTG CCT) present in variable numbers immediately following the coding sequence, is responsible for the length heterogeneity in the spacer region. Some of the gene copies contain a nucleotide change in the coding region which results in a new site for the restriction enzyme Mnl I. The variant 5S RNA produced by these gene copies has not been detected in vivo . Two other single nucleotide variations were identified in the spacer region.Keywords
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