Liquid—liquid extraction of lactate dehydrogenase from muscle using polymer-bound triazine dyes
- 1 August 1986
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 13 (1), 15-27
- https://doi.org/10.1007/bf02798435
Abstract
An extract from porcine muscle containing soluble enzymes has been partitioned between the two liquid phases of an aqueous, biphasic system consisting of dextran, polyethylene glycol, and water. The influence of polymer-bound triazine dyes (Cibacron blue F3G-A and Procion yellow HE-3G) on the partition of lactate dehydrogenase and total protein was studied. The effects of pH and concentrations of polymers and buffer on this so-called affinity partitioning were also determined. The two-phase systems were used in extraction procedures for purification of lactate dehydrogenase to a specific activity of 456–494 U (7.6–8.4 μkat) per mg protein. The use of these systems for extraction of enzymes in technical scale is discussed.Keywords
This publication has 22 references indexed in Scilit:
- Partial purification of d-glucose-6-phosphate dehydrogenase from bakers' yeast by affinity partitioning using polymer-bound triazine dyesEnzyme and Microbial Technology, 1985
- Preparation of cibacron blue F3G‐A (polyethylene glycol) in large scale for use in affinity partitioningBiotechnology & Bioengineering, 1985
- Affinity Partitioning of Phosphofructokinase from Baker's Yeast Using Polymer-Bound Cibacron Blue F3G-AEuropean Journal of Biochemistry, 1983
- Phase Partition—A Method for Purification and Analysis of Cell Organelles and Membrane VesiclesPublished by Wiley ,1982
- Histone‐Hydrocarbon Interaction. Partition of Histones in Aqueous Two‐Phase Systems Containing Poly(ethylene glycol)‐Bound HydrocarbonsEuropean Journal of Biochemistry, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Partition of Proteins in Two‐Phase Systems Containing Charged Poly(ethylene glycol)European Journal of Biochemistry, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The Log S–log z Diagram for Radio Galaxies and its Relation to CosmologyNature, 1970