Cryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity.
Open Access
- 1 June 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (6), 2297-2300
- https://doi.org/10.1073/pnas.74.6.2297
Abstract
Water-soluble polyelectrolytes in interaction with proteins are described. These polyelectrolytes make it possible to investigate enzyme-catalyzed reactions in cooled mixed solvents without the usual effects of their organic solvent component on enzyme specific activity. The applicability of techniques developed is illustrated by results obtained with trypsin. The possibility of an electrostatic "sorting out" of solvents and its potentialities in cryoenzymology are discussed.This publication has 20 references indexed in Scilit:
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