Nucleotide sequence of AKV murine leukemia virus
- 1 February 1984
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 49 (2), 471-478
- https://doi.org/10.1128/jvi.49.2.471-478.1984
Abstract
AKV is an endogenous, ecotropic murine leukemia virus that serves as one of the parents of the recombinant, oncogenic mink cell focus-forming viruses that arise in preleukemic AKR mice. The 8374-nucleotide-long sequence of AKV, as determined from the infecitous molecular clone AKR-623, is reported. The 5''-leader sequence of AKV extends to nucleotide 639, after which lies a long open reading frame encoding the gag and pol gene products. The reading frame is interrupted by a single amber codon separating the gag and pol genes. The pol gene overlaps the env gene within the 3'' region of the AKV genome. The nucleotide sequence of the 5'' region of AKV reveals the following features. The 5''-leader sequence lacks any AUG codon to initiate translation of gPr80gag, suggesting that gPr80gag is not required for the replication of AKV. A short portion of the leader region diverges in sequence from the closely related Moloney murine leukemia virus and appears to be related to a sequence highly repeated in eukaryotic genomes. As in Moloney murine leukemia virus, there is a potential RNA secondary structure flanking the amber codon that separates the gag and pol genes. This structure might function as a regulatory protein binding site that controls the relative levels of synthesis of the gag and pol precursors. The nucleotide sequence of the 3'' region of AKV is compared with sequences reported previously from both infectious and noninfectious molecular clones of AKV.This publication has 65 references indexed in Scilit:
- Cellular origin and role of mink cell focus-forming viruses in murine thymic lymphomasNature, 1982
- Nucleotide sequence of Moloney murine leukaemia virusNature, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses.The Journal of Experimental Medicine, 1980
- The complete sequence of a chromosomal mouse α-globin gene reveals elements conserved throughout vertebrate evolutionCell, 1979
- RNA sequencing provides evidence for allelism of determinants of the N-, B- or NB-tropism of murine leukemia virusesCell, 1979
- Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: Enhancement of the gag-pol polypeptide with yeast suppressor tRNACell, 1978
- A core polyprotein of murine leukemia virus on the surface of mouse leukemia cellsCell, 1976
- STUDIES OF GENETIC TRANSMISSION OF MURINE LEUKEMIA VIRUS BY AKR MICEThe Journal of Experimental Medicine, 1972
- Acceleration of leukemogenesis in akr mice by grafts, cell suspensions, and cell‐free centrifugates of thymuses from preleukemic akr donorsInternational Journal of Cancer, 1968