Analysis of electrical noise in turtle cones

Abstract

Properties of the light-sensitive voltage noise in cones in the retina of the turtle Pseudemys scripta elegans, were studied by intracellular recording. Suppression of the noise by light was a function of the hyperpolarizing response of a cone but not of the size or pattern of illumination. Power density spectra of the noise were fitted in many cones by the product of 2 Lorentzians with characteristics time constants .tau.1 and .tau.2 averaging 40 and 7 ms, respectively. The spectra of some cells were peaked and could be fitted by a resonance curve. Spectra in dim light exhibited decreased low frequency power. They could often be fitted by a product of 2 Lorentzians using the same value of .tau.2 as used in darkness but decreasing .tau.1 and the zero frequency asymptote. An e-fold reduction in .tau.1 occurred with lights which hyperpolarized by 4-7 mV. Injection of hyperpolarizing currents of about 0.1-0.2 nA into weakly coupled cones reduced the noise, and reduced sensitivity to dim flashes. The variance-voltage relation during steady illumination of different intensities differed from cone to cone. Dim lights increased the noise in some cells and decreased it in others, but moderately bright lights which gave steady responses of more than about 1/3 maximal reduced the noise in all cells. When the cell was transiently depolarized during the differentiated component following steady illumination, the noise was less than it was after prolonged darkness. In the after-effect of bright light, the time course of recovery of noise was the same as that of flash sensitivity and voltage. The noise was reduced e-fold for hyperpolarizations averaging 3 mV while for sensitivity this reduction occurred for 1.3 mV. For a given hyperpolarization the noise was lower during the after-effect than during steady dim illumination. When a series of dim flashes was delivered to a cone, no significant increase in variance over the dark noise was detected during the photoresponse. This implies that each photoisomerization evokes no more than about 1.5 .mu.V at the peak of the response in a coupled cone, corresponding to about 50 .mu.V in an isolated cone. The elementary shot events underlying the noise are about 100 .mu.V in amplitude in an isolated cone, have a characteristic time constant of 16-60 ms and reflect unit conductance fluctuations of about 16 pS (Siemen .ident. .OMEGA.-1). The noise source is internal to the cones. Probably, the noise arises from opening and closing of the light-sensitive ionic channels in the outer segment, and in darkness there is a residual concentration of the blocking substance which on average closes up to about 1/3 of the channels. The unit event may involve a considerable number of blocking molecules and ionic channels.