Elongation Factor T from Bacillus stearothermophilus and Escherichia coli
- 1 February 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 62 (2), 373-382
- https://doi.org/10.1111/j.1432-1033.1976.tb10169.x
Abstract
Homogeneous preparations of elongation factors EF-Tu and EF-Ts from B. stearothermophilus were obtained with specific activities of 20,000 .+-. 2000 and 500,000 .+-. 50,000 units/mg, respectively. By dodecylsulfate-polyacrylamide gel electrophoresis the molecular weight of EF-Tu was found to be 49,000 .+-. 2000 and of EF-Ts 35,500 .+-. 1000. Nucleotide-free EF-Tu was prepared by using ITP as a GDP-binding-site-directed analogue. EF-Tu contained 2 sulfydryl groups, 1 reacting fast and 1 slowly with N-ethylmaleimide and 5,5''-dithio-bis(2-nitrobenzoic acid) under non-denaturing conditions. The same reagents reacted with the 3 sulfhydryl groups of EF-Ts in the native state. The heat stabilities of EF-Tu and EF-Ts are reversed with respect to the Escherichia coli factors, EF-Tu being the more stable protein; even nucleotide-free EF-Tu is relatively stable with a half-life at room temperature of about 35 h.This publication has 29 references indexed in Scilit:
- Guanosine triphosphate and guanosine diphosphate as conformation-determining molecules. Differential interaction of a fluorescent probe with the guanosine nucleotide complexes of bacterial elongation factor TuBiochemistry, 1974
- Preliminary X-ray Analysis of the Crystalline Complex between Poypeptide Chain Elongation Factor, Tu, and GDPNature, 1973
- Translation: Its Mechanism and ControlScience, 1972
- Purification of factor Ts: Studies on the formation and stability of nucleotide complexes containing transfer factor TuArchives of Biochemistry and Biophysics, 1971
- Peptide chain elongation. Role of the S1 factor in the pathway form S3-guanosine diphosphate complex to aminoacyl transfer ribonucleic acid-S3-guanosine triphosphate complexBiochemistry, 1971
- A membrane filter assay for protein sulfhydryl groupsBiochemical and Biophysical Research Communications, 1971
- The reactions of the sulfhydryl groups on the elongation factors Tu and TsArchives of Biochemistry and Biophysics, 1971
- Studies on the role of factor Ts in polypeptide synthesisArchives of Biochemistry and Biophysics, 1970
- Crystalline transfer factors from Escherichia coliBiochemical and Biophysical Research Communications, 1968
- Halogenation of tyrosine during acid hydrolysisBiochimica et Biophysica Acta, 1963