Use of Nicotinamide Adenine Dinucleotide (NAD)-Dependent Glucose-6-Phosphate Dehydrogenase in Enzyme Staining Procedures
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 55 (3), 173-176
- https://doi.org/10.3109/10520298009067234
Abstract
Substitution of NAD-dependent glucose-6-phosphate dehydrogenase for the NADP-dependent enzyme has produced identical results in a number of enzyme-linked electrophoretic staining procedures. This substitution significantly reduces the cost of staining for adenylate kinase, creatine kinase, glucosephosphate isomerase, mannosephosphate isomerase, phosphoglucomutase and pyruvate kinase activity by utilizing NAD rather than the more expensive NADP.This publication has 7 references indexed in Scilit:
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