Determination of acetaldehyde in human blood by high-peformance liquid chromatography using fluorometry.
- 1 January 1989
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 37 (1), 200-201
- https://doi.org/10.1248/cpb.37.200
Abstract
A method was developed for the measurement of acetaldehyde in human blood by high-performance liquid chromatography (HPLC). The method was based on a pre-column reaction; a fluorescent substance was formed by a coupling reaction between 2 mol of cyclohexan-1,3-dione and 1 mol of acetaldehyde with ammonium acetate. The couling compound was analyzed by HPLC and the concentration of acetaldehyde was obtained from the calibration curve drawn from the results using standard solutions. The determinations was sensitive and reproducible with a range of 0.2-10 .mu.M and a precision (coefficient of variation) of 2.43%.This publication has 7 references indexed in Scilit:
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