Comparison of multiple HLA-A alleles at the DNA level by using Southern blotting and HLA-A-specific probes.
- 1 December 1985
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 135 (6), 4229-4234
- https://doi.org/10.4049/jimmunol.135.6.4229
Abstract
Numerous alleles of the HLA-A gene have been serologically identified. In this report we present a rapid and straightforward means to assess HLA-A polymorphism at the genomic level. Using 5' and 3' HLA-A-specific DNA probes and Southern blotting, we have placed the recognition sequences for five endonucleases relative to the coding regions of 15 HLA-A alleles. These data permit two interesting conclusions: four of the HLA-A alleles studied are associated with unique restriction fragments, and HLA-A alleles of a cross-reactive group are more closely related at the DNA level than are noncross-reactive alleles.This publication has 1 reference indexed in Scilit:
- Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.Proceedings of the National Academy of Sciences, 1981