Evidence for the presence of cyclic adenosine 3′,5′-monophosphate and its metabolism in loblolly pine (Pinustaeda L.) callus

Abstract

Evidence is presented suggesting the occurrence of cyclic adenosine 3′,5′-monophosphate (cAMP) and adenyl cyclase activity in loblolly pine (Pinustaeda L.) callus cultures grown aseptically on a defined medium. The presence of enzymes in the callus capable of catalyzing cAMP hydrolysis also was detected. Adenosine was the most stable product resulting from cAMP hydrolysis. A direct relationship was found between apparent cAMP concentration and the rate of fresh weight increase of dark-grown callus during a 6-week passage.