Column-Switching High-Performance Liquid Chromatographic Determination of a 2-Pyridinone-Based Human Immunodeficiency Virus Type 1 (HlV-l)-Specific Reverse Transcriptase Inhibitor in Human Plasma
- 1 January 1993
- journal article
- Published by Springer Nature in Pharmaceutical Research
- Vol. 10 (1), 56-60
- https://doi.org/10.1023/a:1018968928742
Abstract
A method for the determination of a 2-pyridinone-based specific HIV-1 reverse transcriptase inhibitor in human plasma is described. Plasma samples are extracted using phenyl solid phase extraction columns. The extract is analyzed via HPLC using a column-switching system to remove interferences from late-eluting endogenous components. Detection is based on UV absorbance at 314 nm. The assay was linear in the concentration range of 10–500 ng/ml, when 1-ml aliquots of plasma were extracted. The mean precision of the assay, expressed as the coefficient of variation, was 3.8%. The assay has been validated and utilized to support human pharmacokinetic studies.Keywords
This publication has 2 references indexed in Scilit:
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- 2-Pyridinone derivatives: a new class of nonnucleoside, HIV-1-specific reverse transcriptase inhibitorsJournal of Medicinal Chemistry, 1991