EXISTENCE OF 2 GAMMA-SUBUNITS OF THE G-PROTEINS IN BRAIN
- 25 September 1989
- journal article
- research article
- Vol. 264 (27), 15758-15761
Abstract
Although amino acid sequences have been determined for the .alpha. and .beta. subunits of Gs, Gi, and Go, sequences have not been reported for the .gamma. subunits of these G proteins. In the present paper, we determined the sequences of peptides prepared by partial proteolysis of two different forms of the .gamma. subunit of Gs, Gi, and Go from bovine brain. Using oligonucleotide probes based on the sequences of two of these peptides, a cDNA clone was isolated from a bovine adrenal cDNA library. This clone contained a 0.9-kilobase cDNA insert that included an open reading frame of 213 bases encoding a 71-amino acid polypeptide with an estimated Mr of 7850. The amino acid sequence predicted for the adrenal cDNA clone was identical to that determined for one form of the .gamma. subunit from brain. In addition, an antibody to a peptide based on the predicted amino acid sequence of this cDNA clone reacted specifically with one of the brain .gamma. subunits, indicating the adrenal cDNA clone encodes a .gamma. subunit present in both adrenal gland and brain. Also, evidence is presented, demonstrating the existence of a second, structurally distinct, form of the .gamma. subunit of Gs, Gi, and Go in brain.This publication has 24 references indexed in Scilit:
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