Destruction of Phosphilipids and Respiratory‐Chain Activity in Pig=Heart Submitochondrial Particles Induced by an Adriamycin‐Iron Complex

Abstract

Ferric ion complexes of the antibiotic and antitumor agent adriamycin support peroxidation of phospholipids in pig heart submitochondrial particles as judged by disappearance of polyunsaturated fatty acids, loss in extractable phospholipid, adramycin-Fe³⁺-deoebdent oxygen consumption and formation of material with ultraviolet absorption. Peroxidation was stimulated to an extent of about 100% at low rates of NADH oxidation in the submitochondrial particles, whereas oxidation rateds higher than 50 nmol NADH oxidized X min⁻¹× mg protein⁻¹ appeared to protect the membrane lipids. NADH oxidase, NADH-cytocyrme c reductase as well as cytochrome c oxidase activities in the submitochondrial particles were progeressively inactivated during incubation with abriamycin in the presence of Fe³⁺ ions, whereas NADH-ferricyanide reductase activity remained fully stable.