The tryptic and chymotryptic fragments of the β‐subunit of guanine nucleotide binding proteins in brain are identical to those of retinal transducin
- 25 March 1985
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 182 (2), 355-359
- https://doi.org/10.1016/0014-5793(85)80332-x
Abstract
The 35-kDa β-subunit of transducin purified from rod outer segment membranes is cleaved into 2 major fragments by trypsin, and 7 major fragments by chymotrypsin. Identical fragments are visualized by immunoblotting with transducin-β specific antisera after proteolysis of rod outer segment membranes, purified brain guanine nucleotide binding proteins, and brain membranes. The results indicate that the β-subunits of transducin and of brain guanine nucleotide binding proteins are not only similar structurally, but are also similarly oriented in membranes with respect to accessibility to proteolytic enzymes.Keywords
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