Unusual Sedimentation and Sulfhydryl Sensitivity of Certain Isohemagglutinins and Skin-Sensitizing Antibody.

Abstract

Comparative sucrose density gradient ultracentrifugation demonstrated that certain human anti-A and anti-B isohemagglutinins, and a human skin-sensitizing antibody reacting with purified glucagon, had sedimentation rates intermediate between those of the classical 7S and 19S antibodies. The intermediately sedimenting isohemagglutinins had sedimentation rates in the range of 9-15S. The skin-sensitizing antibody had a sedimentation rate in the range of 8-11S. The intermediately sedimenting antibodies were recovered on gradient elution DEAE column chromatography after the principle 7S [gamma]-globulin component, but before the majority of 19S antibodies. Both the reaginic skin-sensitizing antibody and the intermediately sedimenting isohemagglutinins were inactivated by treatment with 0. 1[image] mercaptoethanol.