A Single Radioimmunological Assay for Serotonin, N-Acetylserotonin, 5-Methoxytryptamine, and Melatonin
- 1 November 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (5), 1271-1277
- https://doi.org/10.1111/j.1471-4159.1982.tb12565.x
Abstract
A radioimmunoassay of melatonin using a new iodinated derivative has been developed. Simple chemical treatments have then been designed to convert serotonin, N-acetylserotonin, and 5-methoxytryptamine to melatonin. Thus these four molecules, belonging to the same metabolic pathway, were separately assayed in the same radioimmunological system at the same sensitivity level (0.01 pmol). Some biological results on the circadian variations of melatonin and serotonin in blood, pineal, and miscellaneous brain structures are presented.Keywords
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