Oxidative phosphorylation during glycollate metabolism in mitochondria from phototrophic Euglena gracilis
- 1 September 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 150 (3), 373-377
- https://doi.org/10.1042/bj1500373
Abstract
Mitochondria were isolated by gradient centrifugation on linear sucrose gradients from broken cell suspensions of phototrophically grown Euglena gracilis. An antimycin A-sensitive but rotenone-insensitive glycollate-dependent oxygen uptake was demonstrated in isolated mitochondria. The partial reactions of glycollate-cytochrome c oxidoreductase and cytochrome c oxidase were demonstrated by using Euglena cytochrome c as exogenous electron acceptor/donor. Isolated mitochondria contain glycollate dehydrogenase and glyoxylate-glutamate aminotransferase and oxidize exogenous glycine. A P:O ratio of 1.7 was obtained for glycollate oxidation, consistent with glycollate electrons entering the Euglena respiratory chain at the flavoprotein level. The significance of these results is discussed in relation to photorespiration in algae.Keywords
This publication has 21 references indexed in Scilit:
- The determination of inorganic orthophosphate in biological systemsBiochimica et Biophysica Acta (BBA) - General Subjects, 1973
- The Properties and Amino-acid Sequence of Cytochrome c from Euglena gracilisNature, 1973
- Enzymic redox reactions of cytochromes cBiochemical and Biophysical Research Communications, 1972
- Phosphoglycolate production catalyzed by ribulose diphosphate carboxylaseBiochemical and Biophysical Research Communications, 1971
- The intracellular localization of glycollate oxidoreductase in Euglena gracilisBiochemical Journal, 1971
- The occurrence and fine structural characterization of microbodies inEuglena gracilisProtoplasma, 1971
- Ribonucleic acids from the mitochondria of bleached Euglena gracilis z: I. Isolation of mitochondria and extraction of nucleic acidsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
- LEAF PEROXISOMES AND THEIR RELATION TO PHOTORESPIRATION AND PHOTOSYNTHESIS*Annals of the New York Academy of Sciences, 1969
- Oxidative phosphorylation in mitochondria isolated from Euglena gracilisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
- A rapid method for the preparation of highly purified cytochrome oxidaseBiochimica et Biophysica Acta, 1962