Double-antibody method and the protein-A-bearing Staphylococcus aureus cells method compared for separating bound and free antigen in radioimmunoassay.
- 1 May 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (5), 752-756
- https://doi.org/10.1093/clinchem/25.5.752
Abstract
We compared the protein-A-bearing Staphylococcus aureus immunoadsorbent to the double-antibody precipitation method for separating bound and free radiolabeled antigen in a radioimmunoassay. With human albumin (antigen) and rabbit anti-human albumin (antibody) as a model, our results indicate that formalin-fixed, heat-killed S. aureus cells could be substituted for the double-antibody precipitation method. Ease of preparation and high adsorption capacity of protein-A-bearing S. aureus for most mammalian IgG make this method economical and time saving.This publication has 3 references indexed in Scilit:
- Cell Membrane Antigen Isolation with the Staphylococcal Protein A-Antibody AdsorbentThe Journal of Immunology, 1976
- An Improved Method for Isolation of H-2 and Ia Alloantigens with Immunoprecipitation Induced by Protein A-Bearing StaphylococciThe Journal of Immunology, 1976
- IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MANJCI Insight, 1960