Enzyme Multiplied Immunoassay of Phenobarbital by LKB Reaction Rate Analyser: A Comparison with Analysis by Gas-Liquid Chromatography

Abstract
A procedure for the enzyme multiplied immunoassay of phenobarbital using the LKB Reaction Rate Analyser is described. The temperature optimum, linearity, specificity, and precision of the assay are reported. A highly significant correlation coefficient is obtained when the technique is compared with gas-liquid chromatography by analysing human sera containing a range of phenobarbital levels.