PROPERTIES OF AN ANTICOAGULANT FOUND IN THE BLOOD OF A HEMOPHILIAC

Abstract

The anticoagulant had properties similar to those found in 2 other cases, but apparently different than those described for other potential anticoagulants. The anticoagulant prolonged the coagulation time of normal blood. It was stable to heating for 10 min. at 65[degree]C., and over a pH range from 6.5-11.0. It did not show antithrombin activity, was not neutralized by protamine, was not extracted from plasma by ether at neutral pH, and was not dialyzable. It precipitated as a globulin (20% Na sulfite), but was not precipitated as a euglobulin (dilution of the plasma with 10 vols. of distilled water and ad- justment of the pH to 5.5 with acetic acid) and was not adsorbed by aluminum hydroxide gel.