Polyclonal Antibody Synthesis by Bone Marrow Cells of New Zealand Black Mice
- 1 January 1982
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 69 (3), 272-278
- https://doi.org/10.1159/000233183
Abstract
The activity of polyclonal antibody synthesis (PAS) of bone marrow cells (BMC) was assessed by enumerating anti-trinitrophenyl (TNP) plaque-forming cells (PFC) or immunoglobulin-secreting cells (IgSC) generated in the spleen of BMC-reconstituted syngeneic or H-2-compatible hosts. BMC of NZB mice generated large numbers of anti-TNP PFC or IgSC as compared with those of C57BL/6 mice or H-2-compatible, non-autoimmune strains of mice (BALB/c, DBA/2 and B10·D2 mice). Causes for the enhanced PAS activity were explored. Susceptibility to known B cell mitogens was examined and it was shown that spleen cells of the recipients of NZB BMC were refractory to the mitogens while PAS was apparent and acquired responsiveness after 15 days as those from nonautoimmune strains of mice. Furthermore, environmental factor(s) did not seem to be responsible for the enhanced PAS, since BMC from nonautoimmune BALB/c mice showed little PAS activity when transferred into heavily irradiated NZB mice and those of NZB mice showed enhanced PAS activity when transferred into B10·D2 or BALB/c mice. It seems most likely that enhanced PAS activity of BMC of NZB mice is a genetically inherent phenomenon. PAS activity of BMC and spleen cells of NZB mice increased significantly with increasing age of the mice.Keywords
This publication has 21 references indexed in Scilit:
- Immunoregulatory circuits among T-cell sets. II. Physiologic role of feedback inhibition in vivo: absence in NZB mice.The Journal of Experimental Medicine, 1978
- Demonstration of Activation of B Lymphocytes in New Zealand Black Mice at Birth by an Immunoradiometric Assay for Murine IgMThe Journal of Immunology, 1977
- Abnormal Polyclonal B Cell Activation in NZB/NZW F1 MiceThe Journal of Immunology, 1977
- MECHANISM FOR INDUCTION OF ANTI-DNA ANTIBODIES BY BACTERIAL LIPOPOLYSACCHARIDES IN MICE .2. CORRELATION BETWEEN ANTI-DNA INDUCTION AND POLYCLONAL ANTIBODY-FORMATION BY VARIOUS POLYCLONAL LYMPHOCYTE-B ACTIVATORS1977
- Characterization of self-reactive B cells by polyclonal B-cell activators.The Journal of Experimental Medicine, 1977
- Loss of suppressor T cells in adult NZB/NZW mice.The Journal of Experimental Medicine, 1976
- A plaque assay for all cells secreting Ig of a given type or classEuropean Journal of Immunology, 1976
- Decline in Suppressor T Cell Function with Age in Female NZB MiceThe Journal of Immunology, 1974
- Antitrinitrophenyl (TNP) Plaque Assay. Primary Response of Balb/c Mice to Soluble and Particulate ImmunogenExperimental Biology and Medicine, 1969
- FURTHER IMPROVEMENTS IN PLAQUE TECHNIQUE FOR DETECTING SINGLE ANTIBODY-FORMING CELLS1968