Verapamil and Nifedipine Inhibition of Contractions Induced by Potassium and Noradrenaline in Human Mesenteric Arteries and Veins
- 1 February 1979
- journal article
- research article
- Published by Wiley in Acta Pharmacologica et Toxicologica
- Vol. 44 (2), 110-119
- https://doi.org/10.1111/j.1600-0773.1979.tb02304.x
Abstract
Ring preparations of human mesenteric arteries and veins were contracted by noradrenaline (1.8×10-5M) or potassium (127mM). Isometric tension was recorded. In the arterial preparations, the maximum response to noradrenaline was 97±8% (mean±S.E.M.) of that to potassium. In the veins, the corresponding figure was 38±4%. The calcium antagonists verapamil (2.2±10-7-2.2± 10-5M) and nifedipine (2.9×10-8-2.9×10-6M) relaxed both arteries and veins contracted by noradrenaline or potassium, and reduced the responses to these agents when added 15 min. before stimulation. The time course of relaxation of potassium contracted preparations, induced by verapamil and nifedipine, was more rapid and complete than that produced by a calcium-free, high potassium solution. In contrast to verapamil, nifedipine caused a more pronounced inhibition of the potassium than of the noradrenaline evoked contractions in both arteries and veins. After exposure to a calcium-free medium for 30 min., the arterial response to noradrenaline was significantly (P-6M) and nifedipine (2.9-10-7M) were equi-effective in reducing the noradrenaline and potassium induced responses in calcium-free medium. The results suggest that there are differences in reactivity, not only between mesenteric arteries and veins, but also between, e.g. peripheral and mesenteric vessels. The calcium antagonists nifedipine and verapamil do not have an identical mode of action. However, both agents seem to inhibit influx of extracellular calcium, and might also have an inhibitory effect on the release of intracellular calcium.Keywords
This publication has 21 references indexed in Scilit:
- The Effect of Nifedipine on Isolated Human Peripheral VesselsActa Pharmacologica et Toxicologica, 1978
- Effects of Verapamil and Nitroglycerin on Contractile Responses to Potassium and Noradrenaline in Isolated Human Peripheral VeinsActa Pharmacologica et Toxicologica, 1978
- A study of releasable Ca fractions in smooth muscle cells of the rabbit aorta.The Journal of general physiology, 1977
- Specific Pharmacology of Calcium in Myocardium, Cardiac Pacemakers, and Vascular Smooth MuscleAnnual Review of Pharmacology and Toxicology, 1977
- Agonist induced release of intracellular Ca2+ in the rabbit aortaThe Journal of Membrane Biology, 1976
- The effect of verapamil on the response to noradrenaline or to potassium-depolarization in isolated vascular stripsMicrovascular Research, 1974
- Relative contributions of Ca2+ influx and cellular Ca2+ release during drug induced activation of the rabbit aortaPflügers Archiv - European Journal of Physiology, 1974
- The α-adrenoceptor blocking effect of indoramin on human isolated smooth muscleJournal of Pharmacy and Pharmacology, 1973
- Membrane potential and contraction of vascular smooth muscle (portal vein) during application of noradrenaline and high potassium, and selective inhibitory effects of iproveratril (verapamil)Microvascular Research, 1973
- Blockade of Membrane Calcium Fluxes by Lanthanum in Relation to Vascular Smooth Muscle ContractilityArchives Internationales de Physiologie et de Biochimie, 1969