Conditioned insulin secretion and meal feeding in rats.

Abstract

Previous researchers have reported that rats placed upon a feeding regimen such that they receive only 2 h of food per day (meal-fed rats) develop hyperinsulinemia at the time of the day associated with feeding, even in the absence of food. Controls fed ad lib had no such response. In a series of experiments, meal-fed rats had elevated insulin levels at only the specific time of the day associated with feeding, and the increment of insulin at that time could be eliminated with atropine. Free-feeding controls always had higher insulin levels than the meal-fed rats, did not have an elevation of insulin at the time of the day that the meal-fed rats normally ate, and had insulin values that were unaffected by atropine. Hyperinsulinemia can become associated with arbitrary stimuli always associated with eating for meal-fed rats. The hyperinsulinemia of meal-fed rats associated with their feeding time is a learned response.