Abstract
A high pressure liquid chromatographic (HPLC) method has been developed for class fractionating the organic material extracted from atmospheric particulate samples. The procedure involves sample collection on glass fiber filters, Soxhlet extraction of the organic material with cyclohexane, concentration and class fractionation of the organic fraction by adsorption chromatography. The polycyclic aromatic hydrocarbon (PAH) fraction was collected and further fractionated by bonded reverse phase partition chromatography. Benzo[a]- pyrene (B[a]P) was identified and quantitated in this fraction by UV and fluorescence detection.