PRECIPITATION OF CALCIUM PHOSPHATE IN THE HISTOCHEMICAL METHOD FOR PHOSPHATASE
Open Access
- 1 March 1953
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 1 (2), 114-122
- https://doi.org/10.1177/1.2.114
Abstract
Ca phosphate, when precipitated by the addition of phosphate to solutions similar in composition to the histochemical substrate mixture, does not show any experimentally demonstrable tendency to supersaturation. The factors which may contribute to false localizations of enzymatic activity its the Ca-CoS method for alkaline phosphatase are not well understood and certainly not amenable to mathematical analysis for the time being. At present, diffusion artifacts do not appear to be an important source of error, provided incubation time is not unduly extended.Keywords
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