Identification of the Coat Protein Gene of Tomato Golden Mosaic Virus
Open Access
- 1 June 1988
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 69 (6), 1351-1357
- https://doi.org/10.1099/0022-1317-69-6-1351
Abstract
An open reading frame, designated AR1, on DNA component A of tomato golden mosaic virus has been identified as the virus coat protein gene on the basis of the molecular weight and amino acid composition of the virus capsid polypeptide, the mass spectra and N-terminal sequences of peptides produced by cyanogen bromide cleavage of the capsid polypeptide and by the binding of antibodies, induced by immunization of a rabbit with a .beta.-galactosidase-AR1 fusion protein, to the capsid polypeptide in a Western blot.Keywords
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