Covalent Immunoglobulin Assembly in Vitro: Reactivity of Light Chain Covalent Dimers (L 2 ) and Blocked Light Chain Monomers
- 10 February 1978
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 199 (4329), 688-690
- https://doi.org/10.1126/science.415360
Abstract
Covalent light chain dimers (L2) and cysteine-blocked L chain monomers readily react with partially reduced heavy (H) chains. A rapid disappearance of these blocked L chain species is followed by the appearance of covalent intermediates-HL, H2, and H2L-leading to fully assembled H2L2. The mechanism of initial disulfide bond formation between heavy and light chains is disulfide interchange.Keywords
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