Effects of Na+ on the persistence length and excluded volume of T7 bacteriophage DNA

Abstract

Total intensity, Rayleigh light scattering has been used to measure the rms radius, second virial coefficient, persistence length, and excluded volume of homogeneous T7 bacteriophage DNA as a function of Na⁺ concentration (0.005 to 3.0 M). All parameters decrease sharply as [Na⁺] increases, and tend to level off at high Na⁺. The variation of persistence length with [Na⁺] is consistent with predictions from counterion condensation theory.