Expression of human inducible nitric oxide synthase in Escherichia coli
- 29 January 1996
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 379 (2), 135-138
- https://doi.org/10.1016/0014-5793(95)01496-9
Abstract
We have expressed active full-length human inducible nitric oxide synthase (iNOS) in E. coli. Expression required co-expression with calmodulin, a particularly tight-binding cofactor. The extracts also required tetrahydrobiopterin to display activity. Specific activity of the purified recombinant iNOS was similar to iNOS purified from murine macrophages. This result indicates that no special processing events unique to eucaryotic cells are necessary for iNOS activity.Keywords
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