Nitric oxide oxidises a ferrous mammalian lipoxygenase to a pre‐activated ferric species
- 8 July 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 389 (3), 229-232
- https://doi.org/10.1016/0014-5793(96)00591-1
Abstract
Nitric oxide is known as an inhibitor of soybean lipoxygenase-1. Investigating the interaction of a mammalian 15-lipoxygenase with nitric oxide, we found that this enzyme is also inhibited reversibly when incubated with nitric oxide for a short time period (5 s) under anaerobic conditions. This inhibition may be due to the formation of a dissociable lipoxygenase-nitric oxide complex. With longer incubation periods the ferrous lipoxygenase is oxidised to a ferric form. This oxidation renders the enzyme more susceptible to peroxide activation, causing a time-dependent shortening of the kinetic lag phase.Keywords
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