Syngeneic monoclonal antiidiotype can induce cellular immunity to reovirus.

Abstract

A syngeneic monoclonal antiidiotypic antibody was generated in BALB/c mice after repeated immunization with a BALB/c monoclonal anti-reovirus hemagglutinin (HA) antibody. The resultant syngeneic monoclonal antiidiotypic antibody, in the absence of adjuvant, was capable of priming both BALB/c (H-2d, Igh-Ia) and C3H/Hej (H-2k, Igh-Ij) mice for Lyt-1+- and Lyt-2+-dependent responses against the mammalian reovirus. By the use of intertypic reassortants and variant virus analysis, the specificity of the response was finely mapped to the neutralization domain of the viral hemagglutinin (HA). Purified monoclonal antiidiotype was used to compare the potency of antiidiotype to virus in terms of induction of immunity. 8 .times. 108 Protein molecules were able to prime for cellular responses to reovirus. In the reovirus system, T cells and B cells share idiotyic configurations, and antiidiotypic antibodies of the type described may be useful in the development of vaccine against certain viral infections.