Translation of Reovirus Messenger RNAs Synthesized In Vitro into Reovirus Polypeptides by Several Mammalian Cell-Free Extracts
- 1 September 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (9), 2649-2653
- https://doi.org/10.1073/pnas.69.9.2649
Abstract
Single-stranded reovirus RNA, synthesized in vitro by reovirus cores, functioned as messenger RNA in cell-free extracts prepared from several mammalian cells: Krebs II mouse ascites cells, mouse L cells, Chinese hamster ovary cells, HeLa cells, and rabbit reticulocytes. As shown by acrylamide gel electrophoresis, all eight polypeptides known to be specified by reovirus were synthesized in the reticulocyte system. In the other extracts, from 5 to 7 complete virus proteins were made.Keywords
This publication has 30 references indexed in Scilit:
- Messenger activity in mammalian cell-free extracts of reovirus single-stranded RNA prepared in vitroBiochemical and Biophysical Research Communications, 1971
- Tissue-specific Factor required for the Translation of a Mammalian Viral RNANature, 1970
- Initiation of Haemoglobin Synthesis by Methionyl-tRNANature, 1970
- Studies on the intracellular synthesis of reovirus-specified proteinsVirology, 1970
- Translation of RNA from Encephalomyocarditis Virus in a Mammalian Cell-free SystemNature, 1970
- Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus coresVirology, 1969
- Polypeptide components of virions, top component and cores of reovirus type 3Virology, 1969
- Synthesis of Myosin on Heterologous RibosomesCold Spring Harbor Symposia on Quantitative Biology, 1969
- Reovirus: RNA polymerase activity in purified virionsBiochemical and Biophysical Research Communications, 1968
- RNA polymerase activity in purified reoviruses.Proceedings of the National Academy of Sciences, 1968