Mutations to kirromycin resistance occur in the interface of domains I and III of EF‐Tu·GTP
- 26 September 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (2), 118-122
- https://doi.org/10.1016/0014-5793(94)00937-6
Abstract
The antibiotic kirromycin inhibits protein synthesis by binding to EF-Tu and preventing its release from the ribosome after GTP hydrolysis. We have isolated and sequenced a collection of kirromycin resistant tuf mutations and identified thirteen single amino acid substitutions at seven different sites in EF-Tu. These have been mapped onto the 3D structures of EF-Tu·GTP and EF-Tu·GDP. In the active GTP form of EF-Tu the mutations cluster on each side of the interface between domains I and III. We propose that this domain interface is the binding site for kirromycin.Keywords
This publication has 27 references indexed in Scilit:
- Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coliGene, 1992
- Refined structure of elongation factor EF-Tu from Escherichia coliJournal of Molecular Biology, 1992
- Missense substitutions lethal to essential functions of EF-TuBiochimie, 1991
- Kirromycin drastically reduces the affinity of Escherichia coli elongation factor Tu for aminoacyl-tRNABiochemistry, 1991
- Impaired in vitro kinetics of EF‐Tu mutant AaEuropean Journal of Biochemistry, 1990
- Antisuppression by mutations in elongation factor TuEuropean Journal of Biochemistry, 1990
- Mutant forms of tufA and tufB independently suppress nonsense mutationsJournal of Molecular Biology, 1987
- MECHANISM OF ACTION OF KIRROMYCIN-LIKE ANTIBIOTICSAnnual Review of Microbiology, 1985
- Identification of the part of kirromycin structure that acts on elongation factor TuFEBS Letters, 1981
- The role of guanosine 5′-triphosphate in polypeptide chain elongationBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1978