IN VITRO STUDIES OF CELL-BOUND IMMUNITY - CLONING ASSAY OF CYTOTOXIC ACTION OF SENSITIZED LYMPHOID CELLS ON ALLOGENEIC TARGET CELLS
- 1 January 1966
- journal article
- research article
- Vol. 11 (5), 499-+
Abstract
The in vitro reaction between sensitized lymphoid cells and target cells has been studied in an allogeneic transplantation system in mice. Mastocytoma cells of the DBA/2 donor strain were injected into C57BL mice, and the spleens of the recipients harvested 8 days later. The immune lymphoid cells thus obtained were tested for their ability to damage in vitro cultures of target mastocytoma cells. The reaction was followed by 2 methods: microscopic counts, and ability of target cells to form colonies in a semi-solid medium after various periods of contact with immune lymphoid cells. The results show: that the effect on target cells is an exponential function of the number of immune lymphoid cells employed, and that the reaction becomes noticeable after 3 hr. and reaches completion within 12 hr. when evaluated by cloning techniques, while it takes 24-48 hr. to be microscopically clearly demonstrable. Problems arising from use of both methods are discussed.This publication has 11 references indexed in Scilit:
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