QUANTITATIVE ASPECT OF ACID SECRETION RESPONSE OF ISOLATED BULLFROG GASTRIC MUCOSA PREPARATION: EFFECT OF SECRETAGOGUES AND INHIBITORS

Abstract

Isolated Rana catesbeiana gastric mucosa preparations provide experimental advantages for the study of gastric function, as several complex factors affecting gastric secretion can be excluded. The gastric mucosa preparation responded to each stimulant more quickly than did the rat stomach. Accordingly, the effect of the secretagogues in the same preparation could be determined repetitively. The stimulatory effects of tetragastrin, bethanechol and histamine were concentration-dependent and the 3 concentration-response curves were parallel. The actions of tetragastrin or bethanechol were strongly reduced by both atropine and burimamide. The activation of parietal cells by histamine was inhibited only by the histamine H2-receptor antagonist. These fundamentals may provide a reliable basis for discussion of the peripheral mechanism of action of physiological secretagogues and their inhibitors.