Transport of Pyrimidine Nucleosides in Cells of Escherichia coli K 12
Open Access
- 1 November 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 59 (2), 365-372
- https://doi.org/10.1111/j.1432-1033.1975.tb02463.x
Abstract
1 The transport of pyrimidine nucleosides into cells of Escherichia coli has been investigated in mutant strains which cannot metabolize these nucleosides. Such cells transport and concentrate pyrimidine nucleosides several hundred-fold. 2 The transport is inhibited by energy poisons and by sulfhydryl reagents. 3 Pyrimidine nucleosides compete mutually for transport. Adenosine is also a strong competitor while guanosine and inosine are weak competitors. 4 The rate of pyrimidine nucleoside transport is shown to be under control of the cytR and deoR gene products, which are also known to regulate the synthesis of nucleoside-catabolizing enzymes. The transport system is repressed by growth on glucose, as is the synthesis of the enzymes.Keywords
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