Cerebrospinal fluid as a culture medium for human brain tumors

Abstract
Cerebrospinal fluid (CSF) was investigated as a tissue culture medium. Cell cultures derived from tumors of the central nervous system were subcultured in Puck''s medium and in CSF. The rate of growth in CSF was only slightly below that in Puck''s medium. CSF also supported the growth of cells from fresh tumor tissue. Morphologic variations of cells grown in each medium were attributed to their different concentrations of protein.