A new chromatographic method for the determination of thiamine and its mono-, di- and tri-phosphates in animal tissues
- 1 March 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 78 (3), 602-606
- https://doi.org/10.1042/bj0780602
Abstract
A new analytical procedure for the determination of thiamine and its mono-, di- and triphosphoric esters in rat tissue is described. The method allows a mean thiamine recovery of 95%, and good reproducibility. In all the rat tissues studied, thiamine di-phosphate was most abundant thiamine phosphoric ester, but small amounts of thiamine tri- and mono-phosphate were also found.Keywords
This publication has 8 references indexed in Scilit:
- THE QUANTITATIVE HISTOCHEMICAL DISTRIBUTION OF THIAMINE IN NORMAL RAT BRAIN*Journal of Neurochemistry, 1959
- A Study of the Gravimetric Silicotungstate Method of Assay for Thiamin**Ohio State University College of Pharmacy, Columbus 10Journal of the American Pharmaceutical Association (Scientific ed.), 1959
- Manometric determination of cocarboxylase in pure solution, in blood and in animal tissues.1958
- Separation and quantitative determination of thiamine and thiamine phosphoric esters and their preparation in pure formBiochimica et Biophysica Acta, 1954
- Metabolism of thiamine phosphates in washed suspensions of kidney particlesBiochemical Journal, 1954
- On the Presence of the Triphosphothiamine (TPT) in the LiverScience, 1952
- THE MEASUREMENT OF THIAMINE IN URINEJournal of Biological Chemistry, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951