The Destruction of Cytoplasmic Basophilia with Mineral Acids
- 1 January 1953
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 28 (1), 9-12
- https://doi.org/10.3109/10520295309112629
Abstract
Cytoplasmic basophilia may be selectively destroyed by the mineral acids, HNO3, HCl and H2SO4. Their specificity is similar to that of ribonuclease. The optimal conditions for their use are 3°C. for 16 hours at 2M concentrations. Removal of cytoplasmic basophilia as with ribonuclease, malt diastase and perchloric acid is most effective on sections prepared from tissues fixed in solutions containing no chromates. Under the conditions herein reported the mineral acids appear to be a satisfactory and economical substitute for ribonuclease or perchloric acid.Keywords
This publication has 8 references indexed in Scilit:
- Perchloric Acid Extraction of Ribose Nucleic Acid from Cytological PreparationsScience, 1952
- On the destruction of cytoplasmic basophilia (Ribonucleic acid) and of the metachromatic basophilia of cartilage by the glycogen splitting enzyme malt diastase: A histochemical studyThe Anatomical Record, 1949
- The Action of Ribonuclease on Fixed TissuesStain Technology, 1947
- LOCALIZATION OF RIBONUCLEIC ACID IN THE CYTOPLASM OF LIVER CELLSThe Journal of Experimental Medicine, 1946
- PHOSPHORUS COMPOUNDS IN ANIMAL TISSUESJournal of Biological Chemistry, 1945
- PHOSPHORUS COMPOUNDS IN ANIMAL TISSUES .1. EXTRACTION AND ESTIMATION OF DESOXYPENTOSE NUCLEIC ACID AND OF PENTOSE NUCLEIC ACID1945
- Studies in the decalcification of the bone.1944