Time-dependent effects of α-amanitin on blastocyst formation in the mouse
Open Access
- 1 August 1979
- journal article
- research article
- Published by The Company of Biologists in Development
- Vol. 52 (1), 193-202
- https://doi.org/10.1242/dev.52.1.193
Abstract
Early compacting mouse morulae were placed in α-amanitin at various times after HCG and the effect of this transcriptional inhibitor during formation of the blastocyst was noted. No cavitation was observed in those embryos transferred into α-amanitin before 77 h after HCG but the percentage of cavitation increased dramatically in those embryos transferred after 80 h post HCG. The large increase in amino acid incorporation which normally occurs during formation of the blastocyst was blocked in those embryos placed in the inhibitor before 80 h post HCG. Two-dimensional polyacrylamide gel electrophoresis of radiolabelled embryos showed that the changes in synthesis of certain polypeptides normally associated with blastocyst formation did not occur in those embryos placed in α-amanitin before 80 h after HCG. Only one cleavage division appears to occur in the presence of α-amanitin. However, groups of embryos which had completed their fifth division before exposure to the drug subsequently were able to form blastocysts and showed the quantitative and qualitative changes associated with blastocyst formation despite the continued presence of the inhibitor. These results suggest that a critical transcriptional event concerned with blastocyst formation occurs around 80 h after HCG and may be associated with the fifth cleavage division.This publication has 20 references indexed in Scilit:
- Control of protein synthesis during blastocyst formation in the mouseDevelopmental Biology, 1979
- Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by FluorographyEuropean Journal of Biochemistry, 1975
- In vivo and in vitro effect of α-amanitin on preimplantation mouse embryo RNA polymeraseNature, 1974
- The effects of inhibitors of RNA synthesis (α‐amanitin and actinomycin D) on preimplantation mouse embryogenesisJournal of Experimental Zoology, 1973
- Biochemistry of DifferentiationAnnual Review of Biochemistry, 1968
- An Air-Drying Method for Chromosome Preparations from Mouse EggsCytogenetic and Genome Research, 1966
- Protein Synthesis in Enucleated Eggs of Rana pipiensScience, 1965
- Macromolecule synthesis and the influence of actinomycin on early developmentExperimental Cell Research, 1964
- Activation of protein biosynthesis in non-nucleate fragments of sea urchin eggsBiochemical and Biophysical Research Communications, 1963
- Amino acid incorporation into proteins of nucleate and anucleate fragments of sea urchin eggs: Effect of parthenogenetic activationExperimental Cell Research, 1963