Origin of hydrogen in methane produced by Methanobacterium thermoautotrophicum
- 1 February 1980
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 141 (2), 694-698
- https://doi.org/10.1128/jb.141.2.694-698.1980
Abstract
The production of deuterated methane by M. thermoautotrophicum in H2O-D2O mixtures was examined by high-resolution mass spectrometry. The hydrogen in the methane arose solely from water and not from H2 gas. H2 gas served only as an electron source in methanogenesis. A whole-cell product isotope discrimination of 1.5 favoring H2 over deuterium was observed in methane production in 81 atom% deuterated water. The distribution of deuterated methane species is described by a simple model of the overall reaction.This publication has 30 references indexed in Scilit:
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