Tumorigenic Transformation of Primary Rat Embryonal Fibroblasts by Human Papillomavirus Type 8 E7 Gene in Collaboration with the Activated H-ras Gene
Open Access
- 1 December 1991
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 82 (12), 1340-1343
- https://doi.org/10.1111/j.1349-7006.1991.tb01802.x
Abstract
Particular types of human papillomavirus (HPV) are associated with skin cancer of epidermodysplasia verruciformis (EV) patients. Here, we show, for the first time, that the E7 gene of EV-associated HPV8 possesses a potential oncogenic transforming ability. The HPV8 E7 open reading frame (ORF) and the HPV16 E7 ORF were cloned under the SV40 promoter/enhancer to construct recombinant plasmids pcD2-8E7 and pcD2-16E7, respectively. Transfection of primary rat embryonal fibroblasts having an activated H-ras gene revealed that pcD2-8E7 as well as pcD2-16E7 induced transformation of cells in G418-resistant colonies at an efficiency of 12.3% and 42.9%, respectively. The resulting transformed cell lines induced by pcD2-8E7 and activated H-ras were tumorigenic when injected into syngeneic immunocompetent rats. The potential tumorigenicity of HPV8 E7 seemed to be higher than that of HPV16 E7.Keywords
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