Inhibition of mouse T‐cell proliferation by CGRP and VIP: Effects of these neuropeptides on IL‐2 production and cAMP synthesis
- 1 May 1991
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 29 (1), 29-41
- https://doi.org/10.1002/jnr.490290104
Abstract
We compared the effect of two neuropeptides, calcitonin generelated peptide (CGRP) and vasoactive intestinal peptide (VIP), on mitogen-induced murine splenocyte proliferation. Both neuropeptides exerted their maximal effect within 24 hr after activation by Con A. The combination CGRP-VIP caused an additive inhibitory effect on T-cell proliferation. The inhibitory effect of VIP could be correlated with a decrease in interleukin 2 (IL-2) production, whereas CGRP did not affect this production. Since we also observed an additive inhibitory effect on T-cell proliferation by the theophylline and CGRP or VIP combination, we measured the effect of each neuropepntide on intracellular cAMP production by enriched T-cells: CGRP, but not VIP, strongly stimulated cAMP synthesis. Taken together, our results indicate that inhibition of murine T-cell proliferation by CGRP and VIP is mediated by different mechanisms.Keywords
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