Propagation of Conjunctival and HeLa Cells in Various Carbohydrate Media.
- 1 November 1957
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 96 (2), 336-340
- https://doi.org/10.3181/00379727-96-23471
Abstract
Under the described experimental conditions, both conjunctival and Hela cells propagated satisfactorily in the following individual carbohydrates starch, glycogen, maltose, D-glucose, D-fructose, D-mannose and D(+)galactose. Cell propagation in sucrose depended largely on source of dialyzed serum. Sodium pyruvate, sodium lactate, D-ribose andD(+)xylose were unable to support cell multiplication, though cell degeneration in the presence of these carbo-hydrates and intermediates was definitely retarded. L(+)Rhamnose, L(-)fucose and L-sorbose appeared to delay the degeneration of the HeLa cells more effectively than that of the conjunctival cells. Lactose, L(-)xylose, D(-)arabinose, L(+)arabinose, D-2-deoxyribose, i-erythritol and dihydroxyacetone neither supported growth nor retarded degeneration. Glucose at low concentration of 0.1 m[image] is sufficient in supporting cell growth for at least several months. Net increase in number of cells at a concentration of 0.1 m[image] is significantly lower than that in 10 m[image] glucose. When pyruvate and alanine were added to the medium, the concentration of glucose could be further reduced to 0.01 m[image] without resulting in cell degeneration. D-mannose and D(+)galactose both at 0.01 m[image] concentrations, and D-fructose at somewhat higher concentration, are effective substitutes for glucose.Keywords
This publication has 2 references indexed in Scilit:
- THE SPECIFIC AMINO ACID REQUIREMENTS OF A HUMAN CARCINOMA CELL (STRAIN HELA) IN TISSUE CULTUREThe Journal of Experimental Medicine, 1955
- Continuous Subcultivation of Epithelial-like Cells from Normal Human Tissues.Experimental Biology and Medicine, 1954