Phenotypic characterization of human cytolytic T lymphocytes in mixed lymphocyte culture.
Open Access
- 1 January 1981
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 153 (1), 213-218
- https://doi.org/10.1084/jem.153.1.213
Abstract
Mixed lymphocyte reaction (MLR)-activated T cells were analyzed according to the expression of various cell surface markers by the specific cytotoxic T lymphocytes (CTL) generated in the MLR. CTL were found exclusively in a population of MLR-activated T cells that lacked detectable Fc gamma R but that expressed a surface antigen recognized by the 4F2 monoclonal antibody. In contrast, CTL were found in both the Ia-positive and Ia-negative cells after MLR activation. Thus, the specific CTL generated in the allogeneic MLR can be identified and isolated by virtue of the expression of a particular cell surface marker.This publication has 12 references indexed in Scilit:
- Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens.The Journal of Immunology, 1980
- Ia determinants on stimulated human T lymphocytes. Occurrence on mitogen- and antigen-activated T cells.The Journal of Experimental Medicine, 1979
- Insoluble immune complexes suppress mitogen-induced proliferation of human T lymphocytes bearing Fcγ receptorsCellular Immunology, 1979
- Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens.The Journal of Experimental Medicine, 1978
- Loss of Fc receptors for IgG from human T lymphocytes exposed to IgG immune complexesNature, 1978
- Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG.The Journal of Experimental Medicine, 1977
- Subpopulations of Human T Cells Identified by Receptors for Immunoglobulins and Mitogen ResponsivenessThe Journal of Immunology, 1976
- ACTIVATION OF HUMAN B LYMPHOCYTES .2. CELLULAR INTERACTIONS IN PFC RESPONSE OF HUMAN TONSILLAR AND PERIPHERAL-BLOOD B LYMPHOCYTES TO POLYCLONAL ACTIVATION BY POKEWEED MITOGEN1976
- Receptors for IgG molecules on human lymphocytes forming spontaneous rosettes with sheep red cellsEuropean Journal of Immunology, 1975
- GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITROThe Journal of Experimental Medicine, 1974