SINGLE-CELL ISOLATIONS OF DIPLOID HETEROZYGOUS ESCHERICHIA COLI

Abstract

Segregating diploid cultures were studied by a single-cell technic, involving the microscopic observation of the development of clonal pedigrees from single initial cells. The segregation pattern of these cultures was retained through the single-cell isolations so as to preclude the possibility that the diploids represent extracellular associations. The result further confirms the sexual basis of gene recombination in this bacterium.